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CEACAM1/CD66a Recombinant antibody, PBS Only (Capture)

CEACAM1/CD66a Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240497B4

Cat no : 83524-3-PBS

Print datasheet

Synonyms

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

验证数据展示

  • WB analysis using 83524-3-RR (same clone as 83524-3-PBS)

    WB analysis using 83524-3-RR (same clone as 83524-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

    IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

    IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00520-2

    Cytometric bead array standard curve of MP00520-2

    Cytometric bead array standard curve of MP00520-2, CEACAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83524-3-PBS. Detection antibody: 83524-4-PBS. Standard: Eg0314. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP00520-3

    Cytometric bead array standard curve of MP00520-3

    Cytometric bead array standard curve of MP00520-3, CEACAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83524-3-PBS. Detection antibody: 83524-2-PBS. Standard: Eg0314. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83524-3-RR

    Affinity and Kinetic Characterization of 83524-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83524-3-RR against Human CEACAM1 were performed. The affinity constant is 3.58 nM.

产品信息

83524-3-PBS targets CEACAM1/CD66a as part of a matched antibody pair:

MP00520-2: 83524-3-PBS capture and 83524-4-PBS detection (validated in Cytometric bead array)

MP00520-3: 83524-3-PBS capture and 83524-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Synonyms CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1
Calculated Molecular Weight 526 aa, 58 kDa
Observed Molecular Weight135-150 kDa
GenBank Accession NumberBC014473
Gene Symbol CEACAM1
Gene ID (NCBI) 634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13688
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).