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CEBPB Recombinant antibody, PBS Only

CEBPB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240836G6

Cat no : 83791-6-PBS

Print datasheet

Synonyms

CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta

验证数据展示

  • WB analysis using 83791-6-RR (same clone as 83791-6-PBS)

    WB analysis using 83791-6-RR (same clone as 83791-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83791-6-RR (CEBPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 83791-6-RR (same clone as 83791-6-PBS)

    IHC staining of human ovarian cancer using 83791-6-RR (same clone as 83791-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83791-6-RR (CEBPB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CEBPB antibody included
  • IF Staining of HepG2 using 83791-6-RR (same clone as 83791-6-PBS)

    IF Staining of HepG2 using 83791-6-RR (same clone as 83791-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPB antibody (83791-6-RR, Clone: 240836G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83791-6-RR (same clone as 83791-6-PBS)

    FC experiment of HeLa using 83791-6-RR (same clone as 83791-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBPB Recombinant antibody (83791-6-RR, Clone:240836G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83791-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83791-6-RR

    Affinity and Kinetic Characterization of 83791-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83791-6-RR against Human CEBPB were performed. The affinity constant is 69.2 pM.

Planning an IHC experiment? We recommend our IHCeasy CEBPB Ready-To-Use IHC Kit. CEBPB primary antibody included.

产品信息

The immunogen of 83791-6-PBS is CEBPB Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CEBPB fusion protein Ag20073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CCAAT/enhancer binding protein (C/EBP), beta
Synonyms CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta
Calculated Molecular Weight 345 aa, 36 kDa
Observed Molecular Weight42 kDa, 46 kDa
GenBank Accession NumberBC007538
Gene Symbol CEBPB
Gene ID (NCBI) 1051
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17676
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).