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CEBPB Recombinant antibody

CEBPB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240836G6

Cat no : 83791-6-RR

Print datasheet

Synonyms

CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta

验证数据展示

  • WB analysis using 83791-6-RR

    WB analysis using 83791-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83791-6-RR (CEBPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83791-6-RR

    IHC staining of human ovarian cancer using 83791-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83791-6-RR (CEBPB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CEBPB antibody included
  • IF Staining of HepG2 using 83791-6-RR

    IF Staining of HepG2 using 83791-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPB antibody (83791-6-RR, Clone: 240836G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83791-6-RR

    FC experiment of HeLa using 83791-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBPB Recombinant antibody (83791-6-RR, Clone:240836G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83791-6-RR

    Affinity and Kinetic Characterization of 83791-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83791-6-RR against Human CEBPB were performed. The affinity constant is 69.2 pM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, U-87 MG cells, MKN-45 cells, MDA-MB-231 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CEBPB Ready-To-Use IHC Kit. CEBPB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83791-6-RR is CEBPB Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen CEBPB fusion protein Ag20073 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CCAAT/enhancer binding protein (C/EBP), beta
Synonyms CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta
Calculated Molecular Weight 345 aa, 36 kDa
Observed Molecular Weight42 kDa, 46 kDa
GenBank Accession NumberBC007538
Gene Symbol CEBPB
Gene ID (NCBI) 1051
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP17676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).

实验方案

Product Specific Protocols
WB protocol for CEBPB antibody 83791-6-RRDownload protocol
IHC protocol for CEBPB antibody 83791-6-RRDownload protocol
IF protocol for CEBPB antibody 83791-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols