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  • Featured Product
  • KD/KO Validated

CENPF Polyclonal antibody

CENPF Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28568-1-AP

Print datasheet

Synonyms

AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779

验证数据展示

  • WB analysis of HeLa using 28568-1-AP

    WB analysis of HeLa using 28568-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28568-1-AP

    IHC staining of human lung cancer using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 28568-1-AP

    IHC staining of human lung cancer using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 28568-1-AP

    IHC staining of rat small intestine using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 28568-1-AP

    IHC staining of rat small intestine using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 28568-1-AP

    IHC staining of rat testis using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 28568-1-AP

    IF Staining of HeLa using 28568-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse kidney using 28568-1-AP

    IHC staining of mouse kidney using 28568-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 28568-1-AP

    WB analysis using 28568-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inrat small intestine tissue, human lung cancer tissue, mouse liver tissue, mouse kidney tissue, human pancreas cancer tissue, mouse small intestine tissue, rat skin tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28568-1-AP targets CENPF in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen CENPF fusion protein Ag29665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centromere protein F, 350/400ka (mitosin)
Synonyms AH antigen, CENF, CENP F, CENPF, Centromere protein F, hcp 1, Kinetochore protein CENPF, Mitosin, PRO1779
Calculated Molecular Weight 368 kDa
Observed Molecular Weight350 kDa
GenBank Accession NumberNM_016343
Gene Symbol CENPF
Gene ID (NCBI) 1063
RRIDAB_2918176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

实验方案

Product Specific Protocols
WB protocol for CENPF antibody 28568-1-APDownload protocol
IHC protocol for CENPF antibody 28568-1-APDownload protocol
IF protocol for CENPF antibody 28568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Transl Androl Urol

CENPF promotes the proliferation of renal cell carcinoma in vitro

Authors - Ji Zhang
  • KD Validated
View Article
humanWB

Nat Commun

CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes

Authors - Yanfen Zheng
View Article
humanIHC,IF

Oncol Lett

FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma

Authors - Peipei Li
View Article