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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CENPF Polyclonal antibody

CENPF Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28568

Print datasheet

Synonyms

PRO1779, CENP-F, CENF

验证数据展示

  • IF Staining of A549 using CL488-28568

    IF Staining of A549 using CL488-28568

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

  • IF Staining of HeLa using CL488-28568

    IF Staining of HeLa using CL488-28568

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CENPF antibody (CL488-28568) at dilution of 1:200.

  • FC experiment of A549 using CL488-28568

    FC experiment of A549 using CL488-28568

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CENPF Polyclonal antibody (CL488-28568)(red), or 0.4 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inA549 cells, HeLa cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 28568-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28568 targets CENPF in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CENPF fusion protein Ag29665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name centromere protein F, 350/400ka (mitosin)
Synonyms PRO1779, CENP-F, CENF
Calculated Molecular Weight 368 kDa
Observed Molecular Weight350 kDa
GenBank Accession NumberNM_016343
Gene Symbol CENPF
Gene ID (NCBI) 1063
RRIDAB_3084118
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49454
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CENPF antibody CL488-28568Download protocol
FC protocol for CL Plus 488 CENPF antibody CL488-28568Download protocol
Standard Protocols
Click here to view our Standard Protocols