CENPF Recombinant antibody
CENPF Recombinant Antibody for IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
242277G9
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84865-4-RR targets CENPF in IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | CENPF fusion protein Ag29665 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | centromere protein F, 350/400ka (mitosin) |
Synonyms | PRO1779, CENP-F, CENF, 242277G9 |
Calculated Molecular Weight | 368 kDa |
GenBank Accession Number | NM_016343 |
Gene Symbol | CENPF |
Gene ID (NCBI) | 1063 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P49454 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).
实验方案
Product Specific Protocols | |
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IF protocol for CENPF antibody 84865-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |