验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells, A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84865-4-RR targets CENPF in IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | CENPF fusion protein Ag29665 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | centromere protein F, 350/400ka (mitosin) |
别名 | 242277G9, CENF, CENP-F, PRO1779 |
计算分子量 | 368 kDa |
GenBank蛋白编号 | NM_016343 |
基因名称 | CENPF |
Gene ID (NCBI) | 1063 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P49454 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CENPF antibody 84865-4-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |