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CENPF Recombinant antibody, PBS Only

CENPF Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242277G9

Cat no : 84865-4-PBS

Print datasheet

Synonyms

PRO1779, CENP-F, CENF, 242277G9

验证数据展示

  • IF Staining of HeLa using 84865-4-RR (same clone as 84865-4-PBS)

    IF Staining of HeLa using 84865-4-RR (same clone as 84865-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84865-4-RR

    Affinity and Kinetic Characterization of 84865-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

产品信息

84865-4-PBS targets CENPF in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CENPF fusion protein Ag29665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name centromere protein F, 350/400ka (mitosin)
Synonyms PRO1779, CENP-F, CENF, 242277G9
Calculated Molecular Weight 368 kDa
GenBank Accession NumberNM_016343
Gene Symbol CENPF
Gene ID (NCBI) 1063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49454
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).