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CEP135 Monoclonal antibody

CEP135 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C4B7

Cat no : 68715-1-Ig

Print datasheet

Synonyms

CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7

验证数据展示

  • WB analysis using 68715-1-Ig

    WB analysis using 68715-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68715-1-Ig (CEP135 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68715-1-Ig

    IF Staining of HeLa using 68715-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP135 antibody (68715-1-Ig, Clone: 3C4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68715-1-Ig targets CEP135 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CEP135 fusion protein Ag19122 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name centrosomal protein 135kDa
Synonyms CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7
Calculated Molecular Weight 1140 aa, 134 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC136535
Gene Symbol CEP135
Gene ID (NCBI) 9662
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ66GS9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.

实验方案

Product Specific Protocols
WB protocol for CEP135 antibody 68715-1-IgDownload protocol
IF protocol for CEP135 antibody 68715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols