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验证数据展示

  • WB analysis using 68715-1-Ig

    WB analysis using 68715-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68715-1-Ig (CEP135 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68715-1-Ig

    IF Staining of HeLa using 68715-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP135 antibody (68715-1-Ig, Clone: 3C4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

CEP135 Monoclonal antibody

CEP135 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68715-1-Ig

产品说明书

CloneNo. 3C4B7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68715-1-Ig targets CEP135 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CEP135 fusion protein Ag19122 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrosomal protein 135kDa
别名 CEP 135, Centrosomal protein of 135 kDa, centrosomal protein 135kDa, 3C4B7
计算分子量 1140 aa, 134 kDa
观测分子量135 kDa
GenBank蛋白编号BC136535
基因名称 CEP135
Gene ID (NCBI) 9662
RRIDAB_3670408
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ66GS9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.

实验方案

Product Specific Protocols
WB protocol for CEP135 antibody 68715-1-IgDownload protocol
IF protocol for CEP135 antibody 68715-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols