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CEP164 Polyclonal antibody

CEP164 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 22227-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

3F2B7, CEP 164, KIAA1052, centrosomal protein 164kDa, NPHP15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inRPE1 cells
Positive IHC detected inhuman colon tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, A549 cells, PC-3 cells, MDCK cells, hTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22227-1-AP targets CEP164 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, canine
文献引用反应性human, mouse
免疫原 CEP164 fusion protein Ag17570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 164kDa
别名 3F2B7, CEP 164, KIAA1052, centrosomal protein 164kDa, NPHP15
计算分子量 1460 aa, 164 kDa
观测分子量 164 kDa
GenBank蛋白编号BC000602
基因名称 CEP164
Gene ID (NCBI) 22897
RRIDAB_2651175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP164, also called KIAA1052 or NPHP15, is a 1460 amino acid protein containing 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in the G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.

实验方案

Product Specific Protocols
WB protocol for CEP164 antibody 22227-1-APDownload protocol
IHC protocol for CEP164 antibody 22227-1-APDownload protocol
IF protocol for CEP164 antibody 22227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
humanIF

Nat Commun

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Authors - Ning Huang

Nat Commun

Genetic link between renal birth defects and congenital heart disease.

Authors - Jovenal T San Agustin
humanWB

Nat Commun

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Authors - Xiaowei Xu
humanIF

EMBO J

ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.

Authors - Lauren T Evans
mouseIF

Curr Biol

Rab34 is necessary for early stages of intracellular ciliogenesis.

Authors - Michael W Stuck
  • KO Validated
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