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验证数据展示

  • IF Staining of HeLa using CL594-22227

    IF Staining of HeLa using CL594-22227

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 CEP164 antibody (CL594-22227) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CEP164 Polyclonal antibody

CEP164 Polyclonal Antibody for IF/ICC
Cat No. CL594-22227

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

IF/ICC

别名

KIAA1052, CEP 164, 3F2B7, NPHP15, centrosomal protein 164kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 22227-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-22227 targets CEP164 in IF/ICC applications and shows reactivity with human, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, canine
免疫原 CEP164 fusion protein Ag17570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centrosomal protein 164kDa
别名 KIAA1052, CEP 164, 3F2B7, NPHP15, centrosomal protein 164kDa
计算分子量 1460 aa, 164 kDa
观测分子量 164 kDa
GenBank蛋白编号BC000602
基因名称 CEP164
Gene ID (NCBI) 22897
RRIDAB_2919869
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPV0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEP164, also named as KIAA1052 or NPHP15, is a 1460 amino acid protein, which contains 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.

实验方案

Product Specific Protocols
IF protocol for CL594 CEP164 antibody CL594-22227Download protocol
Standard Protocols
Click here to view our Standard Protocols