验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| IF | See 2 publications below |
产品信息
66814-1-Ig targets CEP250,C-NAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | CEP250,C-NAP1 fusion protein Ag5925 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | centrosomal protein 250kDa |
| 别名 | 250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1 |
| 计算分子量 | 281 kDa |
| 观测分子量 | 280 kDa |
| GenBank蛋白编号 | BC001433 |
| 基因名称 | CEP250/CNAP1 |
| Gene ID (NCBI) | 11190 |
| RRID | AB_2882157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BV73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP250,C-NAP1 antibody 66814-1-Ig | Download protocol |
| IF protocol for CEP250,C-NAP1 antibody 66814-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components | ||
Pancreatology Altered centriolar cohesion by CEP250 and appendages impact outcome of patients with pancreatic cancer |