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CEP250,C-NAP1 Monoclonal antibody, PBS Only

CEP250,C-NAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G12D12

Cat no : 66814-1-PBS

Print datasheet

Synonyms

250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1

验证数据展示

  • IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

    IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

  • WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

    WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

产品信息

66814-1-PBS targets CEP250,C-NAP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen CEP250,C-NAP1 fusion protein Ag5925 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name centrosomal protein 250kDa
Synonyms 250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1
Calculated Molecular Weight 281 kDa
Observed Molecular Weight 280 kDa
GenBank Accession NumberBC001433
Gene Symbol CEP250/CNAP1
Gene ID (NCBI) 11190
RRIDAB_2882157
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BV73
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.