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验证数据展示

  • IHC staining of human prostate cancer using 30446-1-AP

    IHC staining of human prostate cancer using 30446-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30446-1-AP (CEP295 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 30446-1-AP

    IHC staining of human prostate cancer using 30446-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30446-1-AP (CEP295 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 30446-1-AP

    IF Staining of U2OS using 30446-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CEP295 antibody (30446-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CEP295 Polyclonal antibody

CEP295 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30446-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

KIAA1731

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30446-1-AP targets CEP295 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CEP295 fusion protein Ag32896 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1731
别名 KIAA1731
计算分子量295 kDa
GenBank蛋白编号NM_033395.1
基因名称 CEP295
Gene ID (NCBI) 85459
RRIDAB_3669722
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C0D2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CEP295 antibody 30446-1-APDownload protocol
IF protocol for CEP295 antibody 30446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols