CEP70 Polyclonal antibody
CEP70 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse testis tissue, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16280-1-AP targets CEP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | CEP70 fusion protein Ag9365 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | centrosomal protein 70kDa |
| Synonyms | p10-binding protein, BITE |
| Calculated Molecular Weight | 597 aa, 70 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC016050 |
| Gene Symbol | CEP70 |
| Gene ID (NCBI) | 80321 |
| RRID | AB_2077083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NHQ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP70, also named as Centrosomal protein of 70 kDa, is a 597 amino acid protein, which directly interacts with tubulin-gamma; this interaction determines centrosomal localization. CEP70 plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, CEP70 is required for the organization and orientation of the mitotic spindle. CEP70 exists some isoforms with MW 70, 67, 65 and 25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP70 antibody 16280-1-AP | Download protocol |
| IHC protocol for CEP70 antibody 16280-1-AP | Download protocol |
| IF protocol for CEP70 antibody 16280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) Identification of biallelic variations of CEP70 in patients with male infertility | ||
