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验证数据展示

  • WB analysis of U-937 using 67079-1-Ig (same clone as 67079-1-PBS)

    WB analysis of U-937 using 67079-1-Ig (same clone as 67079-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67079-1-Ig (CES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)

    IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CES1 antibody included
  • IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)

    IHC staining of human liver using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67079-1-Ig (CES1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CES1 antibody included
  • IF Staining of HepG2 using 67079-1-Ig (same clone as 67079-1-PBS)

    IF Staining of HepG2 using 67079-1-Ig (same clone as 67079-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67079-1-Ig (CES1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67079-1-PBS in a different storage buffer formulation.

CES1 Monoclonal antibody, PBS Only

CES1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67079-1-PBS

产品说明书

CloneNo. 1C11C1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.

产品信息

67079-1-PBS targets CES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 CES1 fusion protein Ag10647 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carboxylesterase 1 (monocyte/macrophage serine esterase 1)
别名 ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase
计算分子量 566 aa, 62 kDa
观测分子量 60-63 kDa
GenBank蛋白编号BC012418
基因名称 CES1
Gene ID (NCBI) 1066
RRIDAB_2882387
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP23141
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).