验证数据展示
经过测试的应用
Positive WB detected in | U-937 cells, THP-1 cells |
Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67079-1-Ig targets CES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | CES1 fusion protein Ag10647 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | carboxylesterase 1 (monocyte/macrophage serine esterase 1) |
Synonyms | ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase |
Calculated Molecular Weight | 566 aa, 62 kDa |
Observed Molecular Weight | 60-63 kDa |
GenBank Accession Number | BC012418 |
Gene Symbol | CES1 |
Gene ID (NCBI) | 1066 |
RRID | AB_2882387 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P23141 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).
实验方案
Product Specific Protocols | |
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WB protocol for CES1 antibody 67079-1-Ig | Download protocol |
IHC protocol for CES1 antibody 67079-1-Ig | Download protocol |
IF protocol for CES1 antibody 67079-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |