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验证数据展示

  • IF Staining of HepG2 using CL594-67079

    IF Staining of HepG2 using CL594-67079

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-67079 (CES1 antibody) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-67079

    IF Staining of HepG2 using CL594-67079

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated CES1 antibody (CL594-67079, Clone: 1C11C1 ) at dilution of 1:100.

CoraLite®594-conjugated CES1 Monoclonal antibody

CES1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67079
CloneNo. 1C11C1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67079-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67079 targets CES1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen CES1 fusion protein Ag10647 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Synonyms ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase
Calculated Molecular Weight 566 aa, 62 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
RRIDAB_2883638
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP23141
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

实验方案

Product Specific Protocols
IF protocol for CL594 CES1 antibody CL594-67079Download protocol
Standard Protocols
Click here to view our Standard Protocols