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验证数据展示

  • IF Staining of HepG2 using CL594-67079

    IF Staining of HepG2 using CL594-67079

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-67079 (CES1 antibody) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-67079

    IF Staining of HepG2 using CL594-67079

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated CES1 antibody (CL594-67079, Clone: 1C11C1 ) at dilution of 1:100.

CoraLite®594-conjugated CES1 Monoclonal antibody

CES1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67079

产品说明书

CloneNo. 1C11C1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CES1 Ready-To-Use IHC Kit. CES1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67079-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67079 targets CES1 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 CES1 fusion protein Ag10647 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carboxylesterase 1 (monocyte/macrophage serine esterase 1)
别名 ACAT, Brain carboxylesterase hBr1, CEH, CES1, CES2, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase
计算分子量 566 aa, 62 kDa
观测分子量 60-63 kDa
GenBank蛋白编号BC012418
基因名称 CES1
Gene ID (NCBI) 1066
RRIDAB_2883638
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP23141
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CES1(liver carboxylesterase 1) is also named as CES2, SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

实验方案

Product Specific Protocols
IF protocol for CL594 CES1 antibody CL594-67079Download protocol
Standard Protocols
Click here to view our Standard Protocols