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CFTR Monoclonal antibody

CFTR Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66928-1-Ig

产品说明书

CloneNo. 2F4A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, ELISA

Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalu-3 cells, HEK-293T cells, HeLa cells, HT-29 cells, Caco-2 cells, MCF-7 cells, MOLT-4 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, A431 cells, A375 cells, A549 cells, NCI-H1299 cells, HepG2 cells
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CFTR Ready-To-Use IHC Kit. CFTR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66928-1-Ig targets CFTR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, rabbit
文献引用反应性mouse
免疫原 CFTR fusion protein Ag27810 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
别名 Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7
计算分子量 168 kDa
观测分子量 150 kDa
GenBank蛋白编号NM_000492
基因名称 CFTR
Gene ID (NCBI) 1080
RRIDAB_2882254
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13569
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.

实验方案

Product Specific Protocols
WB protocol for CFTR antibody 66928-1-IgDownload protocol
IHC protocol for CFTR antibody 66928-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Redox Rep

Ivacaftor attenuates gentamicin-induced ototoxicity through the CFTR-Nrf2-HO1/NQO1 pathway

Authors - Rui Hu
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