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验证数据展示

  • IF Staining of HeLa using CL488-25671

    IF Staining of HeLa using CL488-25671

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CHCHD10 antibody (CL488-25671) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HeLa using CL488-25671

    FC experiment of HeLa using CL488-25671

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CHCHD10 (CL488-25671) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody

CHCHD10 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25671

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, Protein N27C7-4, Protein N27C7 4, N27C7 4, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 25671-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25671 targets CHCHD10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 CHCHD10 fusion protein Ag22598 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 10
别名 CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, Protein N27C7-4, Protein N27C7 4, N27C7 4, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC065232
基因名称 CHCHD10
Gene ID (NCBI) 400916
RRIDAB_3084092
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYQ3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHCHD10 antibody CL488-25671Download protocol
FC protocol for CL Plus 488 CHCHD10 antibody CL488-25671Download protocol
Standard Protocols
Click here to view our Standard Protocols