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  • Featured Product
  • KD/KO Validated

CHCHD2 Monoclonal antibody, PBS Only

CHCHD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1G10

Cat no : 66302-1-PBS

Print datasheet

Synonyms

C7orf17, CHCHD2, NS2TP

验证数据展示

  • WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (66302-1-Ig, Clone: 2G1G10 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
  • IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.

产品信息

66302-1-PBS targets CHCHD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, pig, rat
Immunogen CHCHD2 fusion protein Ag24219 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Synonyms C7orf17, CHCHD2, NS2TP
Calculated Molecular Weight 151 aa, 16 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC003079
Gene Symbol CHCHD2
Gene ID (NCBI) 51142
RRIDAB_2881685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6H1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).