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  • Featured Product
  • KD/KO Validated

CHCHD2 Monoclonal antibody, PBS Only

CHCHD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G1G10

Cat no : 66302-1-PBS

Print datasheet

Synonyms

C7orf17, CHCHD2, NS2TP

验证数据展示

  • WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

    WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (66302-1-Ig, Clone: 2G1G10 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
  • IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.

产品信息

66302-1-PBS targets CHCHD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, pig, rat
Immunogen CHCHD2 fusion protein Ag24219 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 2
Synonyms C7orf17, CHCHD2, NS2TP
Calculated Molecular Weight 151 aa, 16 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC003079
Gene Symbol CHCHD2
Gene ID (NCBI) 51142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6H1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).