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验证数据展示

  • WB analysis using 68259-1-Ig

    WB analysis using 68259-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68259-1-Ig (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 68259-1-Ig

    IF Staining of MCF-7 using 68259-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CHCHD3 antibody (68259-1-Ig, Clone: 1C8H12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human lung using 68259-1-Ig

    IHC staining of human lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHCHD3 antibody included
  • IHC staining of human lung using 68259-1-Ig

    IHC staining of human lung using 68259-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68259-1-Ig (CHCHD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHCHD3 antibody included
  • WB analysis of HeLa using 68259-1-Ig

    WB analysis of HeLa using 68259-1-Ig

    WB result of CHCHD3 antibody (68259-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

CHCHD3 Monoclonal antibody

CHCHD3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68259-1-Ig
CloneNo. 1C8H12
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inpig heart tissue, A549 cells, HeLa cells, rabbit heart tissue, rat heart tissue, mouse heart tissue, rat brain tissue
Positive IHC detected inhuman lung tissue, human lung cancer tissue, mouse cerebellum tissue, mouse lung tissue, rat cerebellum tissue, rat heart tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CHCHD3 Ready-To-Use IHC Kit. CHCHD3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68259-1-Ig targets CHCHD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityrat
Immunogen CHCHD3 fusion protein Ag22628 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Synonyms MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC014839
Gene Symbol CHCHD3
Gene ID (NCBI) 54927
RRIDAB_2935344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NX63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

实验方案

Product Specific Protocols
WB protocol for CHCHD3 antibody 68259-1-IgDownload protocol
IHC protocol for CHCHD3 antibody 68259-1-IgDownload protocol
IF protocol for CHCHD3 antibody 68259-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Ann Transl Med

Electroacupuncture ameliorates surgery-induced spatial memory deficits by promoting mitophagy in rats

Authors - Jian Guo
View Article