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  • Featured Product
  • KD/KO Validated

CHCHD3 Recombinant antibody, PBS Only

CHCHD3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B11

Cat no : 82743-1-PBS

Print datasheet

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

验证数据展示

  • IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

    IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

    WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

    WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

    WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82743-1-RR

    Affinity and Kinetic Characterization of 82743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

Planning an IHC experiment? We recommend our IHCeasy CHCHD3 Ready-To-Use IHC Kit. CHCHD3 primary antibody included.

产品信息

82743-1-PBS targets CHCHD3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CHCHD3 fusion protein Ag22579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Synonyms MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC014839
Gene Symbol CHCHD3
Gene ID (NCBI) 54927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NX63
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.