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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

    WB analysis using 82743-1-RR (same clone as 82743-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

    IF Staining of HepG2 using 82743-1-RR (same clone as 82743-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 82743-1-RR (same clone as 82743-1-PBS)

    IP experiment of Jurkat using 82743-1-RR (same clone as 82743-1-PBS)

    IP result of anti-CHCHD3 (IP:82743-1-RR, 4ug; Detection:82743-1-RR 1:1000) with Jurkat cells lysate 1600 ug. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

    WB analysis of HEK-293 using 82743-1-RR (same clone as 82743-1-PBS)

    WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 82743-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82743-1-RR

    Affinity and Kinetic Characterization of 82743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

  • Featured Product
  • KD/KO验证

CHCHD3 Recombinant antibody, PBS Only

CHCHD3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82743-1-PBS

产品说明书

CloneNo. 4B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CHCHD3 Ready-To-Use IHC Kit. CHCHD3 primary antibody included.

产品信息

82743-1-PBS targets CHCHD3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CHCHD3 fusion protein Ag22579 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 3
别名 MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
计算分子量 227 aa, 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC014839
基因名称 CHCHD3
Gene ID (NCBI) 54927
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX63
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.