验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue |
Positive IP detected in | Jurkat cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82743-1-RR targets CHCHD3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | CHCHD3 fusion protein Ag22579 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | coiled-coil-helix-coiled-coil-helix domain containing 3 |
Synonyms | MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 |
Calculated Molecular Weight | 227 aa, 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC014839 |
Gene Symbol | CHCHD3 |
Gene ID (NCBI) | 54927 |
RRID | AB_3086525 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NX63 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.
实验方案
Product Specific Protocols | |
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WB protocol for CHCHD3 antibody 82743-1-RR | Download protocol |
IF protocol for CHCHD3 antibody 82743-1-RR | Download protocol |
IP protocol for CHCHD3 antibody 82743-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |