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验证数据展示

  • WB analysis using 82743-1-RR

    WB analysis using 82743-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82743-1-RR

    IF Staining of HepG2 using 82743-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of Jurkat using 82743-1-RR

    IP experiment of Jurkat using 82743-1-RR

    IP result of anti-CHCHD3 (IP:82743-1-RR, 4ug; Detection:82743-1-RR 1:1000) with Jurkat cells lysate 1600 ug.

  • WB analysis of HEK-293 using 82743-1-RR

    WB analysis of HEK-293 using 82743-1-RR

    WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82743-1-RR

    Affinity and Kinetic Characterization of 82743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

  • Featured Product
  • KD/KO Validated

CHCHD3 Recombinant antibody

CHCHD3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82743-1-RR
CloneNo. 4B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CHCHD3 Ready-To-Use IHC Kit. CHCHD3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82743-1-RR targets CHCHD3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CHCHD3 fusion protein Ag22579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 3
Synonyms MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
Calculated Molecular Weight 227 aa, 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC014839
Gene Symbol CHCHD3
Gene ID (NCBI) 54927
RRIDAB_3086525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NX63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

实验方案

Product Specific Protocols
WB protocol for CHCHD3 antibody 82743-1-RRDownload protocol
IF protocol for CHCHD3 antibody 82743-1-RRDownload protocol
IP protocol for CHCHD3 antibody 82743-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols