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验证数据展示

  • WB analysis using 82743-1-RR

    WB analysis using 82743-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82743-1-RR (CHCHD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82743-1-RR

    IF Staining of HepG2 using 82743-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (82743-1-RR, Clone: 4B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IP experiment of Jurkat using 82743-1-RR

    IP experiment of Jurkat using 82743-1-RR

    IP result of anti-CHCHD3 (IP:82743-1-RR, 4ug; Detection:82743-1-RR 1:1000) with Jurkat cells lysate 1600 ug.

  • WB analysis of HEK-293 using 82743-1-RR

    WB analysis of HEK-293 using 82743-1-RR

    WB result of CHCHD3 antibody (82743-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD3 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82743-1-RR

    Affinity and Kinetic Characterization of 82743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82743-1-RR against Human CHCHD3 were performed. The affinity constant is 5.97 nM.

  • Featured Product
  • KD/KO验证

CHCHD3 Recombinant antibody

CHCHD3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82743-1-RR

产品说明书

CloneNo. 4B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CHCHD3 Ready-To-Use IHC Kit. CHCHD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82743-1-RR targets CHCHD3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 CHCHD3 fusion protein Ag22579 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 3
别名 MINOS3, MICOS complex subunit MIC19, MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
计算分子量 227 aa, 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC014839
基因名称 CHCHD3
Gene ID (NCBI) 54927
RRIDAB_3086525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX63
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

实验方案

Product Specific Protocols
WB protocol for CHCHD3 antibody 82743-1-RRDownload protocol
IF protocol for CHCHD3 antibody 82743-1-RRDownload protocol
IP protocol for CHCHD3 antibody 82743-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols