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CoraLite®594-conjugated CHCHD6 Monoclonal antibody

CHCHD6 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A11E9

Cat no : CL594-66597

Print datasheet

Synonyms

MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1

验证数据展示

  • IF Staining of MCF-7 using CL594-66597

    IF Staining of MCF-7 using CL594-66597

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 CHCHD6 antibody (CL594-66597, Clone: 2A11E9 ) at dilution of 1:400.

  • FC experiment of A431 using CL594-66597

    FC experiment of A431 using CL594-66597

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated CHCHD6 Monoclonal antibody (CL594-66597, Clone:2A11E9)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of MCF-7 using CL594-66597

    FC experiment of MCF-7 using CL594-66597

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated CHCHD6 Monoclonal antibody (CL594-66597, Clone:2A11E9)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66597-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66597 targets CHCHD6 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CHCHD6 fusion protein Ag14700 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name coiled-coil-helix-coiled-coil-helix domain containing 6
Synonyms MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1
Calculated Molecular Weight 235 aa, 26 kDa
Observed Molecular Weight 26-29 kDa
GenBank Accession NumberBC006123
Gene Symbol CHCHD6
Gene ID (NCBI) 84303
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BRQ6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 CHCHD6 antibody CL594-66597Download protocol
FC protocol for CL594 CHCHD6 antibody CL594-66597Download protocol
Standard Protocols
Click here to view our Standard Protocols