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  • Featured Product
  • KD/KO Validated

CHD4 Monoclonal antibody, PBS Only

CHD4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D9G2

Cat No : 66222-1-PBS

Print datasheet

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA

验证数据展示

  • WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    WB analysis of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    WB result of CHD4 antibody (66222-1-Ig, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66222-1-Ig (same clone as 66222-1-PBS)

    IHC staining of human cervical cancer using 66222-1-Ig (same clone as 66222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHD4 antibody included
  • WB analysis of MCF-7 using 66222-1-Ig (same clone as 66222-1-PBS)

    WB analysis of MCF-7 using 66222-1-Ig (same clone as 66222-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    IF Staining of HEK-293 using 66222-1-Ig (same clone as 66222-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HEK-293 cells using 66222-1-Ig(CHD4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66222-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHD4 Ready-To-Use IHC Kit. CHD4 primary antibody included.

产品信息

66222-1-PBS targets CHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHD4 fusion protein Ag5729 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromodomain helicase DNA binding protein 4
Synonyms ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA
Calculated Molecular Weight 1937 aa, 221 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC038596
Gene Symbol CHD4
Gene ID (NCBI) 1108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14839
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.