验证数据展示
产品信息
66222-1-PBS targets CHD4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, Indirect ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | CHD4 fusion protein Ag5729 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | chromodomain helicase DNA binding protein 4 |
Synonyms | ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA |
Calculated Molecular Weight | 1937 aa, 221 kDa |
Observed Molecular Weight | 260 kDa |
GenBank Accession Number | BC038596 |
Gene Symbol | CHD4 |
Gene ID (NCBI) | 1108 |
RRID | AB_2881613 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q14839 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.