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  • Featured Product
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CHD4 Monoclonal antibody

CHD4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D9G2

Cat no : 66222-1-Ig

Print datasheet

Synonyms

ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA

验证数据展示

  • WB analysis of HEK-293 using 66222-1-Ig

    WB analysis of HEK-293 using 66222-1-Ig

    WB result of CHD4 antibody (66222-1-Ig, 1:1000) with si-Control and si-CHD4 transfected HEK293 cells.

  • IHC staining of human cervical cancer using 66222-1-Ig

    IHC staining of human cervical cancer using 66222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66222-1-Ig (CHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHD4 antibody included
  • WB analysis of MCF-7 using 66222-1-Ig

    WB analysis of MCF-7 using 66222-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66222-1-Ig (CHD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 66222-1-Ig

    IF Staining of HEK-293 using 66222-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HEK-293 cells using 66222-1-Ig(CHD4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CHD4 Ready-To-Use IHC Kit. CHD4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66222-1-Ig targets CHD4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHD4 fusion protein Ag5729 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromodomain helicase DNA binding protein 4
Synonyms ATP dependent helicase CHD4, CHD 4, CHD4, Mi 2b, Mi2 BETA
Calculated Molecular Weight 1937 aa, 221 kDa
Observed Molecular Weight 260 kDa
GenBank Accession NumberBC038596
Gene Symbol CHD4
Gene ID (NCBI) 1108
RRIDAB_2881613
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14839
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

实验方案

Product Specific Protocols
WB protocol for CHD4 antibody 66222-1-IgDownload protocol
IHC protocol for CHD4 antibody 66222-1-IgDownload protocol
IF protocol for CHD4 antibody 66222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
View Article