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验证数据展示

  • IF Staining of HeLa using CL594-66222

    IF Staining of HeLa using CL594-66222

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 CHD4 antibody (CL594-66222, Clone: 3D9G2 ) at dilution of 1:100. Green: Stain with PF00001, CoraLite®488-Phalloidin.

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CoraLite®594-conjugated CHD4 Monoclonal antibody

CHD4 Monoclonal Antibody for IF/ICC
Cat No. CL594-66222

产品说明书

CloneNo. 3D9G2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

ATP dependent helicase CHD4, CHD 4, CHD4, DKFZp686E06161, Mi 2b, Mi2 BETA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CHD4 Ready-To-Use IHC Kit. CHD4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66222-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66222 targets CHD4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 CHD4 fusion protein Ag5729 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromodomain helicase DNA binding protein 4
别名 ATP dependent helicase CHD4, CHD 4, CHD4, DKFZp686E06161, Mi 2b, Mi2 BETA
计算分子量 1937 aa, 221 kDa
观测分子量 260 kDa
GenBank蛋白编号BC038596
基因名称 CHD4
Gene ID (NCBI) 1108
RRIDAB_2919967
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14839
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 CHD4 antibody CL594-66222Download protocol
Standard Protocols
Click here to view our Standard Protocols