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CoraLite®594-conjugated CHD4 Monoclonal antibody
CHD4 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3D9G2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CHD4 Ready-To-Use IHC Kit. CHD4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66222-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66222 targets CHD4 in IF/ICC applications and shows reactivity with human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human |
Immunogen | CHD4 fusion protein Ag5729 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | chromodomain helicase DNA binding protein 4 |
Synonyms | ATP dependent helicase CHD4, CHD 4, CHD4, DKFZp686E06161, Mi 2b, Mi2 BETA |
Calculated Molecular Weight | 1937 aa, 221 kDa |
Observed Molecular Weight | 260 kDa |
GenBank Accession Number | BC038596 |
Gene Symbol | CHD4 |
Gene ID (NCBI) | 1108 |
RRID | AB_2919967 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q14839 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP dependent chromatin remodeler and a major subunit of the repressive NuRD complex [PMID:9790534]. This complex plays key roles in transcriptional regulation and reorganization and maintenance of chromatin structure, as it targets pericentromeric heterochromatin and has recently been implicated in DNA damage repair, being rapidly recruited to DNA double strand breaks [PMID:17694084]. CHD4 is a novel BRIT1 binding partner that regulates the HR repair process, which is a critical pathway of repairing DNA double strand breaks and plays an essential role in maintaining genomic integrity [PMID:22219182]. The calcualted molecular weight of CHD4 is 218 kDa, but modified CHD4 is about 250-280 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 CHD4 antibody CL594-66222 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |