验证数据展示
经过测试的应用
| Positive WB detected in | SGC-7901 cells, K-562 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Planning an IHC experiment? We recommend our IHCeasy CHD9 Ready-To-Use IHC Kit. CHD9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13402-1-AP targets CHD9 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CHD9 fusion protein Ag4245 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromodomain helicase DNA binding protein 9 |
| 别名 | Chromatin-remodeling factor CHROM1, Chromatin-related mesenchymal modulator, CHD-9, ATP-dependent helicase CHD9, AD-013 |
| 计算分子量 | 2897 aa, 326 kDa |
| 观测分子量 | 330 kDa |
| GenBank蛋白编号 | BC033770 |
| 基因名称 | CHD9 |
| Gene ID (NCBI) | 80205 |
| RRID | AB_10640425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3L8U1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IHC protocol for CHD9 antibody 13402-1-AP | Download protocol |
| IF protocol for CHD9 antibody 13402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Carcinog miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.
| ||
Braz J Med Biol Res Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer. | ||
Int J Clin Exp Pathol High CHD9 expression is associated with poor prognosis in clear cell renal cell carcinoma. | ||
mBio A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.
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