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CHKA-Specific Polyclonal antibody

CHKA-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19537-1-AP

Print datasheet

Synonyms

CHETK alpha, CHK, CHKA, CHKA-Specific, Choline kinase alpha, CK, CKI, EK, Ethanolamine kinase;

验证数据展示

  • WB analysis of HepG2 using 19537-1-AP

    WB analysis of HepG2 using 19537-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19537-1-AP (CHKA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19537-1-AP

    IF Staining of HepG2 using 19537-1-AP

    Immunofluorescent analysis of HepG2 cells, using CHKA antibody 19537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human breast cancer using 19537-1-AP

    IHC staining of human breast cancer using 19537-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 10x lens).

  • IHC staining of human breast cancer using 19537-1-AP

    IHC staining of human breast cancer using 19537-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19537-1-AP (CHKA-Specific Antibody) at dilution of 1:50(under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, human testis tissue, mouse colon tissue, mouse testis tissue, Raji cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19537-1-AP targets CHKA-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name choline kinase alpha
Synonyms CHETK alpha, CHK, CHKA, CHKA-Specific, Choline kinase alpha, CK, CKI, EK, Ethanolamine kinase;
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_001277
Gene Symbol CHKA
Gene ID (NCBI) 1119
RRIDAB_10792247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.The antibody is specific to CHKA.

实验方案

Product Specific Protocols
WB protocol for CHKA-Specific antibody 19537-1-APDownload protocol
IHC protocol for CHKA-Specific antibody 19537-1-APDownload protocol
IF protocol for CHKA-Specific antibody 19537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols