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验证数据展示

  • WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

    WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CHMP2B Polyclonal antibody, PBS Only

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12527-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated

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产品信息

12527-1-PBS targets CHMP2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHMP2B fusion protein Ag3222 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromatin modifying protein 2B
Synonyms
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC001553
Gene Symbol CHMP2B
Gene ID (NCBI) 25978
RRIDAB_10603358
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQN3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.