• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

    WB analysis of NIH/3T3 using 12527-1-AP (same clone as 12527-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12527-1-AP (CHMP2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    IHC staining of mouse brain using 12527-1-AP (same clone as 12527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12527-1-AP (CHMP2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    IF Staining of HepG2 using 12527-1-AP (same clone as 12527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CHMP2B antibody (12527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12527-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CHMP2B Polyclonal antibody, PBS Only

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12527-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

12527-1-PBS targets CHMP2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHMP2B fusion protein Ag3222 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromatin modifying protein 2B
别名
计算分子量 24 kDa
观测分子量 32 kDa
GenBank蛋白编号BC001553
基因名称 CHMP2B
Gene ID (NCBI) 25978
RRIDAB_10603358
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQN3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.