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CHMP2B Polyclonal antibody

CHMP2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12527-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

hVps2 2, DMT1, Chromatin-modifying protein 2b, chromatin modifying protein 2B, CHMP2.5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, human heart tissue, mouse brain tissue, human ileum tissue, human kidney tissue, human placenta tissue, human brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, human liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, PFA fixed cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12527-1-AP targets CHMP2B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 CHMP2B fusion protein Ag3222 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromatin modifying protein 2B
别名 hVps2 2, DMT1, Chromatin-modifying protein 2b, chromatin modifying protein 2B, CHMP2.5
计算分子量 24 kDa
观测分子量 32 kDa
GenBank蛋白编号BC001553
基因名称 CHMP2B
Gene ID (NCBI) 25978
RRIDAB_10603358
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQN3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP2B, chromatin-modifying protein 2b, also named CHMP2.5, VPS2B, and VPS2 2, belongs to the chromatin-modifying protein / charged multivesicular body protein (CHMP) family. It is a component of the endosomal sorting complex required for transport III (ESCRT-III), which involves in endosomal and autophagic trafficking of proteins to lysosomes for degradation. Mutations of CHMP2B lead to C-terminal truncation or are replaced with mis-splicing C-termini and cause frontotemporal lobar degeneration (FTLD). In CHMP2B mutation patients, p62- and ubiquitin-positive, but TDP-43 and FUS negative neural inclusions are formed, which may be caused by impaired lysosomal degradation through the autophagy and endosome-lysosome pathways.

实验方案

Product Specific Protocols
WB protocol for CHMP2B antibody 12527-1-APDownload protocol
IHC protocol for CHMP2B antibody 12527-1-APDownload protocol
IF protocol for CHMP2B antibody 12527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Authors - Vincent Mercier
pigWB,IF,IP

PLoS Pathog

Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.

Authors - Chun-Chun Liu
humanIF

Cell Death Dis

iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.

Authors - Kun Gao
mouseWB

J Neurosci

Nascent Aβ42 Fibrillization in Synaptic Endosomes Precedes Plaque Formation in a Mouse Model of Alzheimer's-like β-Amyloidosis

Authors - Elizabeth A Eckman
humanWB

Oncotarget

Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.

Authors - Yi Huo
mouseIF

Sci Rep

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Authors - Carolyn Ott
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