验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HuH-7 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81691-2 targets CHMP4B in WB, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | CHMP4B fusion protein Ag4544 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chromatin modifying protein 4B |
| 别名 | hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178 |
| 计算分子量 | 224 aa, 25 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC033859 |
| 基因名称 | CHMP4B |
| Gene ID (NCBI) | 128866 |
| RRID | AB_3673732 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H444 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 CHMP4B antibody CL750-81691-2 | Download protocol |
| FC protocol for CL Plus 750 CHMP4B antibody CL750-81691-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |