验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HEK-293T cells, mouse spinal cord tissue, rat spinal cord tissue, HeLa cells, Jurkat cells |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16424-1-AP targets CHMP7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, pig |
| 免疫原 | CHMP7 fusion protein Ag9625 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CHMP family, member 7 |
| 别名 | Chromatin-modifying protein 7, Chromatin modifying protein 7 |
| 计算分子量 | 453 aa, 51 kDa |
| 观测分子量 | 51-55 kDa |
| GenBank蛋白编号 | BC019110 |
| 基因名称 | CHMP7 |
| Gene ID (NCBI) | 91782 |
| RRID | AB_2079500 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUX9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHMP7 antibody 16424-1-AP | Download protocol |
| IHC protocol for CHMP7 antibody 16424-1-AP | Download protocol |
| IF protocol for CHMP7 antibody 16424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival | ||
Nat Cell Biol Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.
| ||
Dev Cell CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.
| ||
Dev Cell The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.
| ||
Curr Biol Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.
| ||
