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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CHMP7 Polyclonal antibody

CHMP7 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16424

Print datasheet

Synonyms

Chromatin-modifying protein 7, Chromatin modifying protein 7

验证数据展示

  • IF Staining of A431 using CL488-16424

    IF Staining of A431 using CL488-16424

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CHMP7 antibody (CL488-16424) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 16424-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16424 targets CHMP7 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CHMP7 fusion protein Ag9625 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CHMP family, member 7
Synonyms Chromatin-modifying protein 7, Chromatin modifying protein 7
Calculated Molecular Weight 453 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC019110
Gene Symbol CHMP7
Gene ID (NCBI) 91782
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUX9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHMP7 (Charged multivesicular body protein 7), it is expected to be located in the nucleus and cytoplasm, which is ubiquitinated in lymph node and appendix. It is reported that Lem2p (LEM2), a member of the LEM (Lap2- Emerin-Man1) family of inner nuclear membrane proteins, and the ESCRT-II/ESCRT- III hybrid protein Cmp7p (CHMP7), work together to recruit additional ESCRT-III proteins to holes in the nuclear membrane. CHMP7 protein is involved in several processes, including late endosome to vacuole transport; midbody abscission; and mitotic nuclear division. It is a part of ESCRT III complex, which colocalizes with chromatin. The molecular weight of CHMP7 is 50 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHMP7 antibody CL488-16424Download protocol
Standard Protocols
Click here to view our Standard Protocols