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CHOP; GADD153 Recombinant antibody, PBS Only

CHOP; GADD153 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11

Cat no : 81462-1-PBS

Print datasheet

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

验证数据展示

  • WB analysis of HeLa using 81462-1-RR (same clone as 81462-1-PBS)

    WB analysis of HeLa using 81462-1-RR (same clone as 81462-1-PBS)

    Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81462-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81462-1-PBS

    Affinity and Kinetic Characterization of 81462-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.

产品信息

81462-1-PBS targets CHOP; GADD153 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CHOP; GADD153 fusion protein Ag7354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DNA-damage-inducible transcript 3
Synonyms C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153
Calculated Molecular Weight 19 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35638
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).