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验证数据展示

  • WB analysis of HeLa using 81462-1-RR

    WB analysis of HeLa using 81462-1-RR

    Untreated and tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81462-1-RR (CHOP; GADD153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81462-1-RR

    Affinity and Kinetic Characterization of 81462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81462-1-RR against Human CHOP/GADD153 were performed. The affinity constant is 104 nM.

CHOP; GADD153 Recombinant antibody

CHOP; GADD153 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81462-1-RR

Print datasheet

CloneNo. 1G11

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTunicamycin treated HeLa cells, Tunicamycin treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy DDIT3 Ready-To-Use IHC Kit. DDIT3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81462-1-RR targets CHOP; GADD153 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen CHOP; GADD153 fusion protein Ag7354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DNA-damage-inducible transcript 3
Synonyms C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153
Calculated Molecular Weight 19 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC003637
Gene Symbol CHOP
Gene ID (NCBI) 1649
RRIDAB_2935550
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35638
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

实验方案

Product Specific Protocols
WB protocol for CHOP; GADD153 antibody 81462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols