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CHRNA5 Polyclonal antibody

CHRNA5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13516-1-AP

Print datasheet

Synonyms

CHRNA5, LNCR2, NACHRA5

验证数据展示

  • WB analysis of Jurkat using 13516-1-AP

    WB analysis of Jurkat using 13516-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, A431 cells, A549 cells, mouse bladder tissue, mouse stomach tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13516-1-AP targets CHRNA5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHRNA5 fusion protein Ag4447 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 5
Synonyms CHRNA5, LNCR2, NACHRA5
Calculated Molecular Weight 468 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC033639
Gene Symbol CHRNA5
Gene ID (NCBI) 1138
RRIDAB_2080670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

实验方案

Product Specific Protocols
WB protocol for CHRNA5 antibody 13516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols