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CHRNA5 Monoclonal antibody

CHRNA5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66363-1-Ig

产品说明书

CloneNo. 3G8C11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

WB, IHC, ELISA and More (1)

CHRNA5, LNCR2, NACHRA5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, C2C12 cell, pig skeletal muscle tissue, A549 cells
Positive IHC detected inhuman bladder tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66363-1-Ig targets CHRNA5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, pig
文献引用反应性human, mouse
免疫原 CHRNA5 fusion protein Ag4447 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cholinergic receptor, nicotinic, alpha 5
别名 CHRNA5, LNCR2, NACHRA5
计算分子量 468 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC033639
基因名称 CHRNA5
Gene ID (NCBI) 1138
RRIDAB_2881743
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30532
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

实验方案

Product Specific Protocols
WB protocol for CHRNA5 antibody 66363-1-IgDownload protocol
IHC protocol for CHRNA5 antibody 66363-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Cell Mol Life Sci

Acetylcholine promotes chronic stress-induced lung adenocarcinoma progression via α5-nAChR/FHIT pathway

Authors - Yang Jiao
  • KD Validated
human,mouseIHC

J Invest Dermatol

Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.

Authors - Jing Wang
  • KD Validated
humanIHC

Carcinogenesis

α5-nAChR associated with Ly6E modulates cell migration via TGF-β1/Smad signaling in non-small cell lung cancer.

Authors - Qian Zhang
humanIHC

Mol Carcinog

PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma

Authors - Qiang Li
humanIHC

Toxicology

The role of α5-nicotinic acetylcholine receptor/NLRP3 signaling pathway in lung adenocarcinoma cell proliferation and migration.

Authors - Ying Jia
humanIHC

Hum Cell

The α5-nAChR/PD-L1 axis facilitates lung adenocarcinoma cell migration and invasion.

Authors - Ping Zhu
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