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  • Featured Product
  • KD/KO Validated

CHRNA7 (48 kDa) Recombinant antibody, PBS Only

CHRNA7 (48 kDa) Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A10

Cat no : 82848-3-PBS

Print datasheet

Synonyms

CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10

验证数据展示

  • WB analysis using 82848-3-RR (same clone as 82848-3-PBS)

    WB analysis using 82848-3-RR (same clone as 82848-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-3-RR (CHRNA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 82848-3-RR (same clone as 82848-3-PBS)

    WB analysis of Jurkat using 82848-3-RR (same clone as 82848-3-PBS)

    WB result of CHRNA7 antibody (82848-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells. This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82848-3-RR (same clone as 82848-3-PBS)

    IHC staining of mouse brain using 82848-3-RR (same clone as 82848-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of mouse brain using 82848-3-RR (same clone as 82848-3-PBS)

    IHC staining of mouse brain using 82848-3-RR (same clone as 82848-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of rat dorsal root ganglion using 82848-3-RR (same clone as 82848-3-PBS)

    IHC staining of rat dorsal root ganglion using 82848-3-RR (same clone as 82848-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of rat dorsal root ganglion using 82848-3-RR (same clone as 82848-3-PBS)

    IHC staining of rat dorsal root ganglion using 82848-3-RR (same clone as 82848-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82848-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • Affinity and Kinetic Characterization of 82848-3-RR

    Affinity and Kinetic Characterization of 82848-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-3-RR against Human CHRNA7 (48 kDa) were performed. The affinity constant is 2.04 nM.

Planning an IHC experiment? We recommend our IHCeasy CHRNA7 Ready-To-Use IHC Kit. CHRNA7 primary antibody included.

产品信息

82848-3-PBS targets CHRNA7 (48 kDa) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHRNA7 (48 kDa) fusion protein Ag15980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 7
Synonyms CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10
Calculated Molecular Weight 502 aa, 56 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36544
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)