• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82848-3-RR

    WB analysis using 82848-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-3-RR (CHRNA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 82848-3-RR

    WB analysis of Jurkat using 82848-3-RR

    WB result of CHRNA7 antibody (82848-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells.

  • IHC staining of mouse brain using 82848-3-RR

    IHC staining of mouse brain using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of mouse brain using 82848-3-RR

    IHC staining of mouse brain using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of rat dorsal root ganglion using 82848-3-RR

    IHC staining of rat dorsal root ganglion using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • IHC staining of rat dorsal root ganglion using 82848-3-RR

    IHC staining of rat dorsal root ganglion using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHRNA7 antibody included
  • Affinity and Kinetic Characterization of 82848-3-RR

    Affinity and Kinetic Characterization of 82848-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-3-RR against Human CHRNA7 (48 kDa) were performed. The affinity constant is 2.04 nM.

  • Featured Product
  • KD/KO验证

CHRNA7 (48 kDa) Recombinant antibody

CHRNA7 (48 kDa) Recombinant Antibody for WB, IHC, ELISA
Cat No. 82848-3-RR

产品说明书

CloneNo. 2A10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inrat brain tissue, Jurkat cells
Positive IHC detected inmouse brain tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHRNA7 Ready-To-Use IHC Kit. CHRNA7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82848-3-RR targets CHRNA7 (48 kDa) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHRNA7 (48 kDa) fusion protein Ag15980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cholinergic receptor, nicotinic, alpha 7
别名 CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10
计算分子量 502 aa, 56 kDa
观测分子量~50 kDa
GenBank蛋白编号BC037571
基因名称 CHRNA7
Gene ID (NCBI) 1139
RRIDAB_3670574
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

实验方案

Product Specific Protocols
WB protocol for CHRNA7 (48 kDa) antibody 82848-3-RRDownload protocol
IHC protocol for CHRNA7 (48 kDa) antibody 82848-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols