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  • Featured Product
  • KD/KO Validated

CHRNA7 (48 kDa) Recombinant antibody

CHRNA7 (48 kDa) Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10

Cat No : 82848-3-RR

Print datasheet

Synonyms

CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10

验证数据展示

  • WB analysis using 82848-3-RR

    WB analysis using 82848-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-3-RR (CHRNA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 82848-3-RR

    WB analysis of Jurkat using 82848-3-RR

    WB result of CHRNA7 antibody (82848-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells.

  • IHC staining of mouse brain using 82848-3-RR

    IHC staining of mouse brain using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82848-3-RR

    IHC staining of mouse brain using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat dorsal root ganglion using 82848-3-RR

    IHC staining of rat dorsal root ganglion using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat dorsal root ganglion using 82848-3-RR

    IHC staining of rat dorsal root ganglion using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82848-3-RR

    Affinity and Kinetic Characterization of 82848-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-3-RR against Human CHRNA7 (48 kDa) were performed. The affinity constant is 2.04 nM.

经过测试的应用

Positive WB detected inrat brain tissue, Jurkat cells
Positive IHC detected inmouse brain tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82848-3-RR targets CHRNA7 (48 kDa) in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen CHRNA7 (48 kDa) fusion protein Ag15980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 7
Synonyms CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10
Calculated Molecular Weight 502 aa, 56 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

实验方案

Product Specific Protocols
WB protocol for CHRNA7 (48 kDa) antibody 82848-3-RRDownload protocol
IHC protocol for CHRNA7 (48 kDa) antibody 82848-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols