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CHRNA7 Recombinant antibody, PBS Only

CHRNA7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240117D12

Cat no : 82848-4-PBS

Print datasheet

Synonyms

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12

验证数据展示

  • WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 82848-4-RR (same clone as 82848-4-PBS)

    FC experiment of SH-SY5Y using 82848-4-RR (same clone as 82848-4-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 82848-4-RR (same clone as 82848-4-PBS)

    FC experiment of Jurkat using 82848-4-RR (same clone as 82848-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82848-4-RR

    Affinity and Kinetic Characterization of 82848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy CHRNA7 Ready-To-Use IHC Kit. CHRNA7 primary antibody included.

产品信息

The immunogen of 82848-4-PBS is CHRNA7 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHRNA7 fusion protein Ag15980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 7
Synonyms Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12
Calculated Molecular Weight 502 aa, 56 kDa
Observed Molecular Weight50 kDa, 56 kDa
GenBank Accession NumberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36544
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)