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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    WB analysis using 82848-4-RR (same clone as 82848-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 82848-4-RR (same clone as 82848-4-PBS)

    FC experiment of SH-SY5Y using 82848-4-RR (same clone as 82848-4-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 82848-4-RR (same clone as 82848-4-PBS)

    FC experiment of Jurkat using 82848-4-RR (same clone as 82848-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82848-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82848-4-RR

    Affinity and Kinetic Characterization of 82848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is below 1 pM.

CHRNA7 Recombinant antibody, PBS Only

CHRNA7 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82848-4-PBS

产品说明书

CloneNo. 240117D12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CHRNA7 Ready-To-Use IHC Kit. CHRNA7 primary antibody included.

产品信息

82848-4-PBS targets CHRNA7 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHRNA7 fusion protein Ag15980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cholinergic receptor, nicotinic, alpha 7
别名 Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12
计算分子量 502 aa, 56 kDa
观测分子量50 kDa, 56 kDa
GenBank蛋白编号BC037571
基因名称 CHRNA7
Gene ID (NCBI) 1139
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP36544
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)