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CHRNA7 Recombinant antibody

CHRNA7 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240117D12

Cat No : 82848-4-RR

Print datasheet

Synonyms

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12

验证数据展示

  • WB analysis using 82848-4-RR

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82848-4-RR

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 82848-4-RR

    FC experiment of SH-SY5Y using 82848-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82848-4-RR

    Affinity and Kinetic Characterization of 82848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is 4.96 nM.

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 82848-4-RR is CHRNA7 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CHRNA7 fusion protein Ag15980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cholinergic receptor, nicotinic, alpha 7
Synonyms Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12
Calculated Molecular Weight 502 aa, 56 kDa
Observed Molecular Weight50 kDa, 56 kDa
GenBank Accession NumberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

实验方案

Product Specific Protocols
WB protocol for CHRNA7 antibody 82848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols