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  • Featured Product
  • KD/KO Validated

CHST4 Monoclonal antibody, PBS Only

CHST4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1A4

Cat no : 66623-1-PBS

Print datasheet

Synonyms

CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST

验证数据展示

  • WB analysis of HT-29 using 66623-1-Ig (same clone as 66623-1-PBS)

    WB analysis of HT-29 using 66623-1-Ig (same clone as 66623-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66623-1-Ig (same clone as 66623-1-PBS)

    IHC staining of human lung cancer using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66623-1-Ig (same clone as 66623-1-PBS)

    IF Staining of MCF-7 using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66623-1-Ig (CHST4 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

产品信息

66623-1-PBS targets CHST4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CHST4 fusion protein Ag3987 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
Synonyms CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST
Calculated Molecular Weight 370 aa, 43 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC035282
Gene Symbol CHST4
Gene ID (NCBI) 10164
RRIDAB_2881983
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NCG5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)