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- KD/KO Validated
CHST4 Monoclonal antibody
CHST4 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1E1A4
验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
产品信息
66623-1-Ig targets CHST4 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | CHST4 fusion protein Ag3987 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 |
| Synonyms | CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST |
| Calculated Molecular Weight | 370 aa, 43 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC035282 |
| Gene Symbol | CHST4 |
| Gene ID (NCBI) | 10164 |
| RRID | AB_2881983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NCG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| IHC protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| IF protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genomics Proteomics Bioinformatics miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma. | ||
Front Oncol Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.
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