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验证数据展示

  • IF Staining of HeLa using CL488-66623

    IF Staining of HeLa using CL488-66623

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CHST4 antibody (CL488-66623, Clone: 1E1A4 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated CHST4 Monoclonal antibody

CHST4 Monoclonal Antibody for IF/ICC
Cat No. CL488-66623

产品说明书

CloneNo. 1E1A4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66623-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66623 targets CHST4 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 CHST4 fusion protein Ag3987 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
别名 CHST4, GlcNAc6ST 2, Gn6st 2, GST 3, HEC GlcNAc6ST, LSST
计算分子量 370 aa, 43 kDa
观测分子量 48 kDa
GenBank蛋白编号BC035282
基因名称 CHST4
Gene ID (NCBI) 10164
RRIDAB_2919351
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NCG5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CHST4 antibody CL488-66623Download protocol
Standard Protocols
Click here to view our Standard Protocols