验证数据展示

WB analysis of A549 cells using 10209-2-AP
WB result of CIRBP antibody (10209-2-AP, 1:500) with si-control and si-CIRBP transfected A549 cell.
WB analysis using 10209-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10209-2-AP
IP result of anti-CIRBP (IP:10209-2-AP, 3ug; Detection:10209-2-AP 1:500) with HepG2 cells lysate 6000ug.
IHC staining of human pancreas cancer using 10209-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CIRBP antibody included
IHC staining of human pancreas cancer using 10209-2-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CIRBP antibody included
IF Staining of HEK-293 using 10209-2-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CIRBP antibody (10209-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of A549 using 10209-2-AP
1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CIRBP (10209-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

CIRBP Polyclonal antibody

CIRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10209-2-AP

Publications(82)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

Synonyms

CIRP, A18HNRNP, A18 hnRNP

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (82)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	Y79 cells, A549 cells, HEK-293 cells, HepG2 cells, PC-12 cells, PC-13 cells, mouse testis tissue, rat testis tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human pancreas cancer tissue, human breast cancer tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	A549 cells

Planning an IHC experiment? We recommend our IHCeasy CIRBP Ready-To-Use IHC Kit. CIRBP primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 20 publications below
WB	See 67 publications below
IHC	See 14 publications below
IF	See 12 publications below
IP	See 2 publications below
CoIP	See 1 publications below
RIP	See 2 publications below

产品信息

10209-2-AP targets CIRBP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, RIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, squirrel
Immunogen	CIRBP fusion protein Ag0280 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	cold inducible RNA binding protein
Synonyms	CIRP, A18HNRNP, A18 hnRNP
Calculated Molecular Weight	172 aa, 19 kDa
Observed Molecular Weight	19 kDa
GenBank Accession Number	BC000403
Gene Symbol	CIRBP
Gene ID (NCBI)	1153
RRID	AB_2080263
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14011
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

实验方案

Product Specific Protocols
WB protocol for CIRBP antibody 10209-2-AP	Download protocol
IHC protocol for CIRBP antibody 10209-2-AP	Download protocol
IF protocol for CIRBP antibody 10209-2-AP	Download protocol
IP protocol for CIRBP antibody 10209-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nature RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration. Authors - Diego Peretti View Article
human,squirrel	WB	Cell iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. Authors - Jingxing Ou View Article
rat	WB	Nat Med Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis. Authors - Qiang Xiaoling X View Article
human	WB	Mol Cell Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection. Authors - Nandan S Gokhale View Article
human	WB	Nucleic Acids Res Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection Authors - Morgane Corre View Article
human	WB,IHC	J Exp Clin Cancer Res Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression Authors - Lixia Li KD Validated View Article

验证数据展示

WB analysis of A549 cells using 10209-2-AP

WB analysis using 10209-2-AP

IP experiment of HepG2 using 10209-2-AP

IHC staining of human pancreas cancer using 10209-2-AP

IHC staining of human pancreas cancer using 10209-2-AP

IF Staining of HEK-293 using 10209-2-AP

FC experiment of A549 using 10209-2-AP

CIRBP Polyclonal antibody

CIRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.

Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression