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验证数据展示

  • FC experiment of A549 using CL594-10209

    FC experiment of A549 using CL594-10209

    1X10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human CIRBP (CL594-10209) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HEK-293 using CL594-10209

    IF Staining of HEK-293 using CL594-10209

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 CIRBP antibody (CL594-10209) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated CIRBP Polyclonal antibody

CIRBP Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-10209

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

CIRP, A18HNRNP, A18 hnRNP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CIRBP Ready-To-Use IHC Kit. CIRBP primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10209 targets CIRBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CIRBP fusion protein Ag0280 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cold inducible RNA binding protein
别名 CIRP, A18HNRNP, A18 hnRNP
计算分子量 172 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC000403
基因名称 CIRBP
Gene ID (NCBI) 1153
RRIDAB_2934699
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14011
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

实验方案

Product Specific Protocols
IF protocol for CL594 CIRBP antibody CL594-10209Download protocol
FC protocol for CL594 CIRBP antibody CL594-10209Download protocol
Standard Protocols
Click here to view our Standard Protocols