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验证数据展示

  • FC experiment of HeLa using CL647-16006

    FC experiment of HeLa using CL647-16006

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human CISD1 (CL647-16006) and HRP-conjugated polymer at dilution 1:1000 (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated CISD1 Polyclonal antibody

CISD1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-16006

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

CISD1, Cysteine transaminase CISD1, EC:2.6.1.3, MDS029, MitoNEET, ZCD1, C10orf70, CDGSH iron sulfur domain 1, CDGSH iron-sulfur domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16006-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-16006 targets CISD1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 CISD1 fusion protein Ag8680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CDGSH iron sulfur domain 1
别名 CISD1, Cysteine transaminase CISD1, EC:2.6.1.3, MDS029, MitoNEET, ZCD1, C10orf70, CDGSH iron sulfur domain 1, CDGSH iron-sulfur domain-containing protein 1
计算分子量 108 aa, 12 kDa
观测分子量 14-17 kDa
GenBank蛋白编号BC007043
基因名称 CISD1
Gene ID (NCBI) 55847
RRIDAB_2934922
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZ45
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CISD1 antibody CL647-16006Download protocol
Standard Protocols
Click here to view our Standard Protocols