- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated CISD1 Polyclonal antibody
CISD1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 16006-1-AP
推荐稀释比
Application | Dilution |
---|---|
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-16006 targets CISD1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | CISD1 fusion protein Ag8680 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CDGSH iron sulfur domain 1 |
Synonyms | CISD1, Cysteine transaminase CISD1, EC:2.6.1.3, MDS029, MitoNEET, ZCD1, C10orf70, CDGSH iron sulfur domain 1, CDGSH iron-sulfur domain-containing protein 1 |
Calculated Molecular Weight | 108 aa, 12 kDa |
Observed Molecular Weight | 14-17 kDa |
GenBank Accession Number | BC007043 |
Gene Symbol | CISD1 |
Gene ID (NCBI) | 55847 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NZ45 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.
实验方案
Product Specific Protocols | |
---|---|
FC protocol for CL Plus 647 CISD1 antibody CL647-16006 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |