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  • Featured Product
  • KD/KO Validated

CISD2 Polyclonal antibody

CISD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13318-1-AP

Print datasheet

Synonyms

MitoNEET related 1 protein, Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2

验证数据展示

  • WB analysis using 13318-1-AP

    WB analysis using 13318-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13318-1-AP

    IHC staining of human liver cancer using 13318-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 13318-1-AP

    IP experiment of mouse brain using 13318-1-AP

    IP result of anti-CISD2 (IP:13318-1-AP, 3ug; Detection:13318-1-AP 1:700) with mouse brain tissue lysate 4000ug.

  • IF Staining of HepG2 using 13318-1-AP

    IF Staining of HepG2 using 13318-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (13318-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse brain tissue, mouse heart tissue, human brain tissue, rat kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13318-1-AP targets CISD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, drosophila
Immunogen CISD2 fusion protein Ag4172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDGSH iron sulfur domain 2
Synonyms MitoNEET related 1 protein, Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2
Calculated Molecular Weight 135 aa, 15 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC032300
Gene Symbol CISD2
Gene ID (NCBI) 493856
RRIDAB_2080270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5K1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on the endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates the autophagy program by interacting with BCL2, contributing to antagonizing BECN1-mediated cellular autophagy at the endoplasmic reticulum.

实验方案

Product Specific Protocols
WB protocol for CISD2 antibody 13318-1-APDownload protocol
IHC protocol for CISD2 antibody 13318-1-APDownload protocol
IF protocol for CISD2 antibody 13318-1-APDownload protocol
IP protocol for CISD2 antibody 13318-1-APDownload protocol
FC protocol for CISD2 antibody 13318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, mouse, ratWB

Proc Natl Acad Sci U S A

A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.

Authors - Simin Lu
  • KD Validated
View Article
WB

Elife

Sampling the conformational space of the catalytic subunit of human γ-secretase.

Authors - Xiao-Chen Bai
View Article
humanWB

EMBO Rep

Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.

Authors - Diana Molino
  • KD Validated
View Article
humanWB,IHC

Oncotarget

Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

Authors - Lan Wang
  • KD Validated
View Article
mouseWB,IHC

FASEB J

A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.

Authors - Michael Curley
  • KO Validated
View Article
humanWB,IHC

Oncotarget

A novel prognostic score model incorporating CDGSH Iron Sulfur Domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

Authors - Lin Yang
View Article