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CoraLite® Plus 647-conjugated CISD2 Polyclonal antibody

CISD2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13318

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Synonyms

MitoNEET related 1 protein, Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2



经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13318-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.00 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13318 targets CISD2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CISD2 fusion protein Ag4172 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CDGSH iron sulfur domain 2
Synonyms MitoNEET related 1 protein, Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2
Calculated Molecular Weight 135 aa, 15 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC032300
Gene Symbol CISD2
Gene ID (NCBI) 493856
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5K1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CISD2 antibody CL647-13318Download protocol
Standard Protocols
Click here to view our Standard Protocols